通过现场电序测绘绘制分子和功能细胞状态的多模式图
Qiang Li1, Zuwan Lin2, Ren Liu1
1John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA 02134, USA.
Cell
|April 20, 2023
概括
我们开发了现场电序测 (electro-seq) 来绘制同一个细胞中的电活动和基因表达. 这种方法揭示了心脏和神经组织中的基因与电生理学关系.
科学领域:
- 生物物理
- 分子生物学
- 系统生物学
背景情况:
- 了解电源组织中的基因与功能关系需要同时测量单细胞水平上的电活动和基因表达.
- 现有的方法往往缺乏从完整网络中的相同细胞绘制两种模式的分辨率或能力.
研究的目的:
- 开发和验证一种新的技术,即现场电序测定 (electro-seq),用于在完整的生物网络中对单细胞的电活动和基因表达进行配对的多模式分析.
- 在人类诱导的多能干细胞衍生心肌细胞的发育过程中揭示基因电生理学关系的实用性.
主要方法:
- 现场电序测 (electro-seq) 集成了灵活的生物电子与现场RNA测序.
- 这种技术可以稳定地绘制毫秒时间尺度的电活动,并同时对单细胞基因表达进行分析.
- 该方法应用于完整的心脏和神经补丁,包括人类诱导的多能干细胞衍生的心肌细胞补丁.
主要成果:
- 在细胞水平上进行心肌细胞电生理学和基因表达的多模式实地分析.
- 这项研究共同确定了心肌细胞补丁中的细胞状态和发育轨迹.
- 在整个心肌细胞发育过程中,基于机器学习的交叉模式分析确定了基因与电生理学的关键关系.
- 基因表达特征的演变是从长期电气测量中准确地重建的.
结论:
- 在现场的电测序为电源组织的时空多模式映射提供了强大的工具.
- 这种技术有助于发现新的细胞类型和基因程序的基础电生理功能和功能障碍.
- 在复杂的生物系统中更深入地了解基因与功能关系.
相关概念视频
01:18Molecular Shapes
63.4K
Molecules have characteristic shapes that are crucial for their function. The arrangement of various electron groups around the central atom dictates their molecular geometry. Electron pairs in the valence shell of a central atom will adopt an arrangement that minimizes repulsions between the electron pairs by maximizing the distance between them. The valence electrons form either bonding pairs, located primarily between bonded atoms, or lone pairs.
Two regions of electron density in a diatomic...
Two regions of electron density in a diatomic...
63.4K
02:00Molecular Models
45.6K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
45.6K
03:51Molecular Orbital Theory II
28.5K
Molecular Orbital Energy Diagrams
28.5K
02:42Valence Bond Theory
11.7K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
11.7K
02:40MO Theory and Covalent Bonding
14.9K
The molecular orbital theory describes the distribution of electrons in molecules in a manner similar to the distribution of electrons in atomic orbitals. The region of space in which a valence electron in a molecule is likely to be found is called a molecular orbital. Mathematically, the linear combination of atomic orbitals (LCAO) generates molecular orbitals. Combinations of in-phase atomic orbital wave functions result in regions with a high probability of electron density, while...
14.9K
01:21Cell Diagrams and IUPAC Conventions
108
Electrochemical cell notation is a standardized symbolic representation that communicates the structure and reaction pathway of galvanic and electrolytic cells. This notation plays a critical role in describing redox reactions and electrochemical cell configurations without the need for detailed diagrams.In electrochemical cell notation, a single vertical line “|” denotes a phase boundary, such as between a solid electrode and an aqueous solution. A double vertical line...
108