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Updated: Aug 1, 2025

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

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生物直角连接增强活细胞中G蛋白结合受体的药物片段亲和力

Jordan M Mattheisen1,2, Chris Limberakis3, Roger B Ruggeri3

  • 1Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, New York, New York 10065, United States.

Journal of the American Chemical Society
|April 28, 2023
PubMed
概括

研究人员开发了一种将药物片段与G蛋白合受体 (GPCRs) 结合的新方法,从而提高对这些重要点的药物发现. 这一策略在基于细胞的系统中改进了基于片段的GPCR查.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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