在疹病毒凝聚物中因应激活的分子机制
Xiaojie Zhang1, Sindhuja Sridharan2, Ievgeniia Zagoriy1
1Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, Germany.
Cell
|April 28, 2023
概括
细胞应激可以重新激活持久性疹病毒感染. 这项研究揭示了压力如何通过改变细胞凝聚物中的病毒蛋白动态和基因组可访问性来触发病毒复制.
科学领域:
- 病毒学
- 细胞生物学
- 结构生物学
背景情况:
- 负链RNA病毒产生持久性感染,通过细胞内包容引起慢性疾病.
- 在持久性感染中,宿主和病毒之间的平衡是微妙的,可以被破坏.
研究的目的:
- 研究细胞应激如何破坏宿主病毒平衡,并诱导疹病毒的复制.
- 了解压力诱导病毒重新激活的分子机制.
主要方法:
- 采用细胞生物学,全细胞蛋白质组学和冷电子断层扫描.
- 开发了疹病毒核体的原子模型.
主要成果:
- 持续的病毒复制工厂是由病毒蛋白驱动的动态蛋白-RNA凝聚物.
- 细胞压力增加了蛋白酸化,稳定了病毒复制复合体.
- 这种稳定导致核体的结构变化,暴露了病毒基因组.
结论:
- 细胞应激作为一个开关,调节病毒凝聚物以提高持久性疹病毒的复制率.
- 病毒蛋白是压力中介病毒重新激活的关键调节剂.
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