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Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

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Updated: Jul 31, 2025

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

合成细胞内模式生物计算的正交光激活DNA

Denis Hartmann¹, Razia Chowdhry¹, Jefferson M Smith¹

¹Department of Chemistry, University of Oxford, Mansfield Road, Oxford OX1 3TA, U.K.

Journal of the American Chemical Society

|May 1, 2023

概括

研究人员开发了一种新的蓝光激活DNA, 该系统独立于紫外线工作,使合成细胞的双波长控制成为先进的生物应用.

科学领域:

合成生物学
生物技术
分子生物学

背景情况:

无细胞基因表达对于研究生物系统至关重要,并具有生物技术潜力.
复杂的通路和合成细胞需要精确控制无细胞系统中的DNA模板.
像可见光这样的远程无害刺激提供了先进的控制方法.

研究的目的:

合成蓝光可激活的DNA部分,用于调节无细胞RNA和蛋白质合成.
使用蓝光独立于紫外线 (UV) 光来实现基因表达的正交控制.
创建一个双波长光控制的无细胞AND门,用于精确的时空调节.

主要方法:

蓝光可激活DNA结构的合成.
用先前开发的紫外线激活DNA测试直角性.
构建和测试无电池 AND 门逻辑电路.
将直角DNA部分封装成合成细胞.
应用重叠的蓝光和紫外线图案进行时空控制.

主要成果:

成功合成了可以通过蓝光激活的DNA部分来调节细胞自由表达.
在蓝色和紫外线激活DNA之间证明基因表达的直角启动.

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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相关实验视频

Last Updated: Jul 31, 2025

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

设计了一种双波长光控制的无细胞AND门.

通过重叠的光模式,在合成细胞中实现了对 AND 门的精确时空控制.

结论:

开发了新的蓝色和紫外线直角光可激活的DNA系统.
这些系统能够精确地控制无细胞基因表达.
在合成生物学,无细胞系统和医学中开辟了先进控制的新可能性.