人类肠道病毒的结构图谱
Oliver W Bayfield1, Andrey N Shkoporov2, Natalya Yutin3
1York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK. oliver.bayfield@york.ac.uk.
Nature
|May 3, 2023
概括
使用冷电子显微镜揭示了大量人类肠道病毒的结构. 这项研究发现了一种新的
科学领域:
- 微生物学
- 结构生物学
- 病毒学
背景情况:
- CrAssphage和相关病毒 (crassviruses) 是人类肠道微生物群中最为丰富的病毒.
- 它们在微生物组合和功能中的重要作用已得到认可,但病毒蛋白质结构和功能在很大程度上仍未知.
- 之前的知识依赖于通用的生物信息预测.
研究的目的:
- 确定一个突出的人类肠道病毒的结构, Bacteroides intestinalis病毒 ΦcrAss001.
- 为赋予蛋白的功能提供结构基础.
- 为了阐明crassvirus组装和感染的机制.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来重建 ΦcrAss001 病毒结构.
- 生物信息分析与功能分配的结构数据相结合.
- 对体和尾部内的蛋白质定位进行了比较分析.
主要成果:
- 获得了FcrAss001的高分辨率冷电磁结构.
- 发现了一种独特的"粗折叠"的大型口蛋白组件,
- 在体和尾部观察到大量的载荷蛋白储存,有证据表明蛋白质喷射机制涉及展开.
结论:
- 这项研究提供了对crassvirus架构和蛋白质功能的第一个结构见解.
- 已发现的"粗折叠"和货物喷射机制为了解这些丰富的肠道病毒提供了基础.
- 这些发现对于了解crassvirus对人类肠道微生物群的影响至关重要.
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