一个酸盐感应器官调节酸盐和组织平衡
Chiwei Xu1,2, Jun Xu3,4, Hong-Wen Tang3,5
1Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. charlesxu.harvard@gmail.com.
Nature
|May 3, 2023
概括
通过降低PXo传递器的调节,Drosophila中的酸盐饥饿会导致消化组织过度生长. 这导致通常储存酸盐的PXo体的降解,增加细胞质水平并触发信号级联.
科学领域:
- 细胞生物学
- 分子生物学
- 生理学
背景情况:
- 动物组织中的细胞内无机酸盐 (Pi) 代谢和信号传递仍然不太清楚.
- 长期的Pi饥饿已被观察到在Drosophila melanogaster的消化表皮中诱导过度增殖.
研究的目的:
- 调查Pi输送器PXo在Pi稳态中的作用及其对组织增殖的影响.
- 阐明Pi饥饿影响细胞Pi水平和信号通路的机制.
主要方法:
- 有改变PXo表达的Drosophila melanogaster模型.
- 免疫覆盖和细胞器的超结构分析.
- 基于福斯特共振能量转移 (FRET) 的Pi成像.
- PXo体的蛋白质和脂质特征.
- 对JNK信号通路的分析.
主要成果:
- 的饥饿会降低传递器PXo的调节,导致中肠的过度扩散.
- PXo标志着非正规多层细胞器官 (PXo体) 的生物发生,它们作为细胞内Pi储备.
- PXo限制细胞质Pi水平,而其在细胞质Pi饥饿期间的降解会增加细胞质Pi.
- 基因酶与AP-1 (Cka) 的连接器通过JNK信号传递调解Pi饥饿诱导的过度增殖.
结论:
- PXo体是细胞内Pi水平的关键调节者.
- 一个涉及PXo,Cka和JNK的Pi依赖信号级联控制组织平衡,以响应Pi的可用性.
更多相关视频
08:07Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Published on: July 26, 2019
8.6K
08:49Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
4.1K
相关概念视频
Roles of Electrolytes: Calcium and Phosphate
345
Calcium and phosphate are essential electrolytes in the human body, with calcium being the most abundant mineral. Around 99% of the body's calcium is stored in the skeleton and teeth, forming a crystal lattice of mineral salts in combination with phosphates. Calcium plays crucial roles in various bodily functions such as blood clotting, neurotransmitter release, muscle tone maintenance, and nervous and muscle tissue excitability.
The calcium concentration in blood plasma is primarily...
The calcium concentration in blood plasma is primarily...
345
Phosphate Buffer
1.8K
The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
1.8K
pH Regulation in Cells
6.2K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
6.2K
Introduction to Electrolytes
10.4K
In humans, electrolytes play a vital role in various physiological processes. Balancing electrolyte levels is essential for normal body functions; their imbalance can be life-threatening. The major electrolytes include sodium, potassium, chloride, calcium, phosphate, and bicarbonate. They are primarily involved in physiological processes, such as nerve signal transmission, membrane trafficking, muscle contraction, buffering body fluids, and balancing water levels in the body.
Role of Sodium
One...
Role of Sodium
One...
10.4K
Skeleton and Calcium Homeostasis
4.6K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.6K
Phosphorylation
50.6K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
50.6K
