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从3D胚芽细胞培养中获得的Cynomolgus子胚胎的神经

Jinglei Zhai¹, Yanhong Xu¹, Haifeng Wan¹

¹State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China.

|May 12, 2023

概括

研究人员为子胚胎开发了一种新的3D培养系统,使得早期神经发育和神经管缺陷的研究成为可能. 这项突破提供了关于灵长类胚胎形成和出生缺陷的潜在原因的见解.

科学领域:

发育生物学
干细胞生物学
基因组学

背景情况:

神经管 (NT) 缺陷是全球常见的出生缺陷,但灵长类神经发育机制尚不清楚.
研究的局限性包括对人类胚胎研究的禁止和模型系统的不足.

研究的目的:

建立非人类灵长类动物胚胎发育的3D延长体外培养 (pIVC) 系统.
在实验室中研究早期胚胎形成,化和神经管形成.

主要方法:

开发了一种3D长期体外培养 (pIVC) 系统,用于子胚胎 (受精后7-25天).
使用单细胞多组分析和免疫光.
将体外胚胎发育与体内胚胎和人类胚胎进行比较.

主要成果:

pIVC胚胎成功地形成了三个胚胎层和原始胚胎细胞.
通过胃化确立了正确的DNA甲基化和染色质可访问性.
证实了神经形成,神经关闭和神经前区域化.
在活体灵长类胚胎中反映的转录特征和形态遗传学.

结论:

通过先进的胃化和早期的神经化,pIVC系统支持非人类灵长类胚胎的发育.
这种模型为研究灵长类胚胎和神经管缺陷提供了新的途径.

关键词:

子子早期的胚胎发育消化过程在体外培养神经母体区域化关闭神经管神经形成非人类灵长类动物单细胞多组学研究三个细菌层

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