两光子合成孔径显微镜用于深层组织中原生亚细胞行为的最小侵入性快速3D成像
Zhifeng Zhao1, Yiliang Zhou1, Bo Liu2
1Department of Automation, Tsinghua University, Beijing 100084, China; Institute for Brain and Cognitive Sciences, Tsinghua University, Beijing 100084, China; Beijing Key Laboratory of Multi-dimension & Multi-scale Computational Photography (MMCP), Tsinghua University, Beijing 100084, China; IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China; Hangzhou Zhuoxi Institute of Brain and Intelligence, Hangzhou 311100, China.
Cell
|May 13, 2023
概括
这项研究引入了一种新的双光子显微镜 (TPM) 技术,增强了深层组织成像. 这种先进的方法减少了光漂白,并提高了分辨率,使得体内细胞下动态和生物过程的详细可视化.
科学领域:
- 生物医学成像
- 细胞生物学
- 神经科学
背景情况:
- 对生理病理过程的整体理解需要先进的3D成像技术.
- 目前的双光子显微镜 (TPM) 由于扫描方法和光毒性而面临时空分辨率,成像体积和持续时间的限制.
- 光学偏差进一步挑战使用TPM进行深层组织成像.
研究的目的:
- 克服传统的深层组织成像TPM的局限性.
- 在毫秒尺度上实现异常校正的亚细胞动态3D成像.
- 为了实现长期的大容量体内成像,减少光漂白.
主要方法:
- 在双光子显微镜中利用合成光圈雷达原理.
- 开发了用于深层组织可视化的偏差校正成像方法.
- 通过大批量 (>100,000) 实现了亚细胞动态的毫秒级成像.
主要成果:
- 与传统的TPM相比,光漂白减少了三倍.
- 在深层组织中成功成像了亚细胞动态,并增强了时空分辨率.
- 通过创伤后脑损伤产生直接的细胞间通信.
- 视觉化小鼠淋巴结中的生殖中心形成和小鼠视觉皮层中特征化的细胞状态.
结论:
- 开发的TPM技术显著提升了静脉内成像能力.
- 这种方法通过将亚细胞行为与生理生成联系起来,从而促进对生物系统的整体理解.
- 开辟了研究复杂生物组织和功能中的新途径.
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