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Updated: Jul 30, 2025

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

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电磁共旋-CaV结构显示了一个离子通道组装中间体

Zhou Chen1, Abhisek Mondal1, Fayal Abderemane-Ali1,2

  • 1Cardiovascular Research Institute, University of California, San Francisco, CA, USA.

Nature
|May 17, 2023
PubMed
概括

细胞内膜膜蛋白复合体 (EMC) 作为一个伴侣,帮助组装电压离子通道 (VGIC). 结构洞察力揭示了EMC如何促进CaV通道的形成及其与辅助子单元的相互作用.

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
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Sec61 protein conducting channel
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