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相关概念视频

Types of RNA01:23

Types of RNA

64.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.9K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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相关实验视频

Updated: Jul 29, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

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将非活性RNA结合小分子编程为生物活性降解剂

Yuquan Tong1, Yeongju Lee1, Xiaohui Liu1

  • 1Department of Chemistry, The Scripps Research Institute & The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, USA.

Nature
|May 24, 2023
PubMed
概括
此摘要是机器生成的。

研究人员研究了小分子如何与RNA相互作用, 他们开发了一种使用这些结合剂降解向RNA的新方法,成功向与疾病相关的microRNA-155和mRNA.

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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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相关实验视频

Last Updated: Jul 29, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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科学领域:

  • 分子生物学
  • 医学化学
  • 有关RNA疗法

背景情况:

  • 小分子与RNA结合是实现生物活动的挑战.
  • 了解RNA与小分子相互作用对于药物发现至关重要.

研究的目的:

  • 研究小分子和3DRNA结构之间的分子识别模式.
  • 使用小分子调节RNA生物学的策略.
  • 开发用于治疗的RNA向降解剂.

主要方法:

  • 研究了以天然产品为灵感的小分子集合和折叠的RNA结构之间的相互作用.
  • 在人类转录组中绘制了RNA-小分子相互作用景观.
  • 设计用于向核糖酶的嵌合体来诱导RNA裂变.
  • 验证了前体微RNA-155,JUN mRNA和MYC mRNA的降解剂.

主要成果:

  • 确定了RNA与小分子相互作用的结构-活性关系.
  • 由于结合位置,大多数相互作用被预测为生物惰性.
  • 证明不活跃的结合剂可以转化为强大的RNA降解剂.
  • 成功设计了针对特定RNA目标的选择性降解剂.

结论:

  • 可以利用小分子-RNA相互作用进行向的RNA降解.
  • 向核糖酶的嵌合体提供了一个将弱结合物转化为RNA功能强大的调节器的策略.
  • 这种方法对开发新型RNA向疗法具有前景.