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在南非以前未接受过治疗的患者中,肝炎C病毒基因型5的变异性
Tshegofatso K Maunye1,2, Maemu P Gededzha3, Jason T Blackard4,5
1HIV and Hepatitis Research Unit, Department of Virology, Sefako Makgatho Health Sciences University, Pretoria, South Africa.
Intervirology
|May 26, 2023
概括
抗性相关变异 (RAVs) 在南非的型肝炎病毒 (HCV) 基因型5感染中很常见. 对于这些患者,建议在治疗前进行耐药性测试.
科学领域:
- 病毒学 病毒学
- 遗传学 是一个遗传学.
- 流行病学 流行病学
背景情况:
- 型肝炎病毒 (HCV) 基因型5在南非普遍存在,占感染的35-60%.
- 关于南非HCV5基因型病例中耐药性相关变异 (RAV) 的数据有限.
- 这项研究集中在比勒陀利亚的乔治·穆卡里博士学术医院 (DGMAH) 的未经治疗的个人身上.
研究的目的:
- 研究HCV基因型5.5的NS3/NS4A,NS5A和NS5B基因中的遗传变异性和确定RAV.
- 评估与这一群体中抗病毒耐药性相关的特定突变的流行率.
主要方法:
- 嵌套PCR被用来放大目标基因 (NS3/4A,NS5A,NS5B).
- 用Geno2pheno工具对已识别的RAV进行评估.
- 对从未接受过治疗的患者的样本进行了测序和分析.
主要成果:
- 在NS3/4A中发现了F56S和T122A突变 (每个样本1个),在7个样本中发现了D168E.
- 在NS5A中检测到T62M突变在2个个体中.
- 在NS5B中,67%的人有A421V,100%的人有S486A突变.
结论:
- 在南非,常常在未接受过治疗的5型HCV基因组患者中检测到RAV.
- 在开始治疗前对基因型5感染进行抗药性测试是建议的.
- 需要进一步的基于人口的研究来确定这些RAV的总体流行率.
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