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牙面膜的类分析 - 考古学,人类学或法医研究的性别估计工具

Ivan Mikšík¹, Marine Morvan^1,2, Jaroslav Brůžek³

¹Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.

Journal of separation science

|May 26, 2023

概括

蛋白质组学提供了一种可靠,具有成本效益的方法,用于使用牙面膜蛋白估计性别,克服了古老样本中DNA分析的局限性. 这种蛋白质分析方法对于法医和人类学研究至关重要.

科学领域:

法医科学法医科学法医科学
生物化学生化学
人类学是人类学.

背景情况:

用于人类和动物物质识别的DNA分析受到放大问题,污染,成本和DNA降解的限制.
骨科,基因组学和蛋白质组学是目前用于性别估计的方法,但它们在应用法医和人类学环境中的比较可靠性尚未得到充分证实.

研究的目的:

评价蛋白质组学作为一种可靠,非破坏性和具有成本效益的性别估计方法.
评估牙牙中阿米洛根因蛋白质异型在法医和人类学背景下用于性别确定的潜力.

主要方法:

利用蛋白质组学,特别是液体染色学和质谱学,检测牙面膜中的性别特异的杏仁蛋白异型 (Y和X).
分析蛋白质在硬牙组织 (牙质) 中的保存情况,以便在古老样本中应用.

主要成果:

蛋白质组学为DNA分析提供了一种无污染的替代性,用于性别估计.
蛋白质,如阿梅洛金,在牙面膜中长时间 (几万年) 保存.
氨基基基因Y异型仅在男性乳液中存在,而氨基基基因X异型在两性中都存在.

结论:

蛋白质组学,特别是牙面膜中的阿梅洛金因分析,为估计性别提供了一个有希望,具有成本效益和最小破坏性的方法.
这种技术对于考古学,人类学和法医应用非常有价值,特别是当DNA分析具有挑战性或不可能时.

关键词:

考古学考古学考古学考古学性别的确定性性别的确定性牙的牙质是牙上的牙质.

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