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益生菌调节丸基因表达和细胞循环过程,主要通过JAK2/STAT5途径在雄性大鼠中
Sanketa Raut1, Kushaan Khambata2, Vincent Goffin3
1Department of Neuroendocrinology, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai 400012, India.
Endocrinology
|May 26, 2023
概括
益生菌在男性生殖中起着关键作用. 这项研究揭示了益生菌受体的激活,并确定了由大鼠丸中益生菌调节的关键基因,影响精子生成和生育能力.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 超性乳腺血症影响高达16%的不育男性.
- 益生素受体 (PRLR) 在精子生成中的生理作用尚未完全理解.
- 在大鼠丸中,青春期和成年期期间,益生菌和PRLR的表达增加.
研究的目的:
- 为了研究大鼠丸组织中益生素的生理作用.
- 为了确定PRLR在丸中激活的信号通路.
- 为了确定男性生殖中由益生菌蛋白对基因转录的调节.
主要方法:
- 测量血清前素和丸PRLR表达的测量.
- 对与PRLR相关的信号通路 (JAK2/STAT5,MAPK/ERK,PI3K/AKT) 的分析.
- 精子管培养中的基因表达概况.
- 对差异表达基因的定量PCR (qPCR) 验证.
主要成果:
- 在丸细胞中,PRLR显著激活JAK2/STAT5通路.
- 益生菌治疗改变了692个基因的表达,包括那些参与细胞循环和男性繁殖的基因.
- 六个细胞周期基因被上调,四个基因被益生菌抑制.
- 鉴定和验证了丸中的新型益生菌向基因.
结论:
- 益生菌在男性生殖中起着至关重要的作用.
- JAK2/STAT5通路是丸中益生菌信号传递的关键媒介.
- 益生菌调节特定的参与细胞循环,染色质重塑和细胞骨组织的基因,影响精子生成.
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