在非稳定状态下进行Laisk测量:在暴露于变暖和可变CO2度的植物中进行测试
Stephanie C Schmiege1,2, Thomas D Sharkey1,3,4, Berkley Walker3,5
1Plant Resilience Institute, Michigan State University, East Lansing, MI 48824, USA.
Plant physiology
|May 26, 2023
概括
动态同化技术 (DAT) 提供了一种更快的方法来测量植物中的光呼吸 (RL) 和细胞间CO2 (Ci*). 虽然DAT和稳定状态方法产生了类似的RL,但DAT显示了更高的Ci*值,可能是由于光吸变化.
科学领域:
- 植物生理学 植物生理学
- 光合作用研究研究光合作用.
- 气体交换分析
背景情况:
- 光呼吸 (RL) 对于植物碳平衡和光合作用建模至关重要.
- 传统的莱斯克方法用于RL测量需要稳定状态条件.
- 动态同化技术 (DAT) 可能可以实现更快的RL估计.
研究的目的:
- 评估DAT用于估计RL和细胞间CO2 (Ci*) 的有效性.
- 为了比较DAT测量与传统的稳定状态方法.
- 调查环境因素 (温度,二氧化碳) 对RL和Ci*估计的影响.
主要方法:
- 对RL和Ci*估计的DAT和稳定状态Laisk方法的比较.
- 在纸树 (Betula papyrifera) 上在不同温度和CO2下进行的实验.
- 进一步比较DAT估计的RL和Ci*在混合 (Populus nigra L. × P. maximowiczii A. Henry"NM6") 与不同的二氧化碳预处理.
主要成果:
- DAT和稳定状态方法在纸树中产生了可比的RL估计值.
- 观察到RL对温度升高或CO2的最小适应.
- 使用DAT与稳定状态方法相比,Ci*估计结果始终更高.
- 二氧化碳预处理放大了DAT和稳定状态测量之间的Ci*差异.
结论:
- DAT是快速RL测量的一种可行的替代方案.
- 在DAT和稳定状态方法之间观察到的Ci*差异可能与光呼吸过程中的甘氨酸出口有关.
- 环境因素,如二氧化碳预处理,可能会影响Ci*测量,强调需要仔细的实验设计.
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