基因组学和现象学使预育种成为可能,改善了 Sorghum 中早期季节的冷却耐受性
Sandeep Marla1, Terry Felderhoff1, Chad Hayes2
1Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.
G3 (Bethesda, Md.)
|May 26, 2023
概括
在中发展冷却耐受性 (CT) 允许早期种植,以提高作物产量. 先进的现象学和基因组学成功将CT培育成精英,在寒冷压力下增强苗木的性能.
科学领域:
- 农业科学 农业科学
- 植物育种 植物育种
- 基因组学就是基因组学.
背景情况:
- 在温带地区早些时候种植热带作物提供了诸如延长生长季节和减少水损失等好处.
- ,一个热带作物,表现出冷却敏感性,阻碍了早期种植. 由于相关的不良特征,传统育种一直在努力进入冷却耐受性 (CT).
研究的目的:
- 为了利用现象学和基因组学在中预育种早期季节冷却耐受性 (CT).
- 开发和验证分子标记物,以有效地对CT进行内侵.
主要方法:
- 使用无人机系统 (UAS) 进行高通量表型化,以识别CT的定量特征位置 (QTL).
- 利用种群基因组学和固定指数分析来识别在供体系中的罕见CT等位基因.
- 开发并测试了用于标记器辅助育种的竞争性等位基特定PCR (KASP) 标记物.
主要成果:
- 无人驾驶系统的表型与手动方法有中等的相关性,并确定了CT QTL.
- 第一代KASP标记器的实用性有限,而来自人口基因组学的第二代标记器是成功的.
- 标记器辅助育种将CT从中国入美国精英品种,在冷却压力下提高了13-24%的苗木性能.
结论:
- 高通量表型化和种群基因组学对于繁殖复杂的适应性特征,如谷CT.有效.
- CT的成功内进可以提高季节早期的表现,使得在温带气候下能够更广泛地种植.
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