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Updated: Jun 14, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Published on: April 8, 2017

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用GeneXpert®系统检测到的SARS-CoV-2的循环值 (Ct) 和与不同目标基因失败相关的突变

Keita Yamashita1, Terumi Taniguchi1, Noriyasu Niizeki1

  • 1Department of Laboratory Medicine, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.

Current issues in molecular biology
|May 26, 2023
PubMed
概括

在SARS-CoV-2测试原始体中的特定突变在医院COVID-19爆发期间导致不准确的结果. 将基因之间的周期值进行比较,提高了病毒传播的准确性和理解.

关键词:
在Ct值中,值为t.G29179TT 这是一个很好的方法.基因Xpert 是一个专家.这就是SARS-CoV-2病毒.感染控制的方法质量控制质量控制质量控制

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...