相关实验视频
Updated: Jul 29, 2025
09:47
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
19.1K
在实验性牙周炎的牙上皮细胞和巨细胞中Wnt信号激活
1Department of Immunology and Infectious Diseases, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA.
Dentistry journal
|May 26, 2023
概括
在巨细胞中异常的Wing/Wnt信号激活在牙周炎期间促进炎症. 准Wnt通路可能为这种炎症性牙疾病提供新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 口腔生物学 口腔生物学
背景情况:
- 无翼/集成 (Wnt) 信号对平衡至关重要,并与炎症性疾病有关.
- 在牙周炎期间,Wnt信号在巨细胞中的具体作用尚不清楚.
研究的目的:
- 在实验性牙周炎中研究Wnt信号与巨细胞之间的相互作用.
- 确定牙周炎期间巨细胞中Wnt信号传递的促炎作用.
主要方法:
- 在小鼠中使用 Porphyromonas gingivalis (P.g) 结合剂诱导实验性牙周炎.
- 免疫组织化学评估了牙周组织中TNF-α,β-catenin和F4/80的表达.
- 西方斑点分析了Wnt对TNF-α在Raw 264.7巨细胞和牙上皮细胞 (GEC) 的信号效应.
主要成果:
- 观察到TNF-α和活性β-catenin的过度表达在牙周炎受影响的牙周的巨细胞中.
- 在巨细胞中,Wnt通路的激活增加了TNF-α,但在GEC中没有.
- P.g脂聚糖 (LPS) 诱导巨细胞中的Wnt信号元件,被DKK1.1阻断.
结论:
- 在实验性牙周炎期间,巨细胞中异常激活了翅膀/Wnt信号.
- 在巨细胞中激活的Wnt信号可能有助于牙周炎的炎症过程.
- 准Wnt通路为牙周炎治疗提供了潜在的治疗途径.
相关概念视频
02:54Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
01:41Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K