在患有细胞和肝细胞癌的患者中测量卡博赞提尼布血水平,使用适合临床应用的简单HPLC-UV方法
Takeo Yasu1,2, Yoshito Gando1, Ryosuke Nishijima1
1Department of Medicinal Therapy Research, Pharmaceutical Education and Research Center, Meiji Pharmaceutical University, Tokyo 204-8588, Japan.
监测血中的卡博桑提尼布水平对于管理癌症治疗至关重要. 这项研究提出了一种快速的HPLC-UV方法来准确量化卡博桑提尼布,有助于临床患者监测.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 卡博桑提尼布治疗细胞和肝细胞癌.
- 与卡博赞提尼布相关的不良事件是剂量依赖的.
- 治疗药物监测可以优化卡博桑提尼布治疗的有效性和安全性.
研究的目的:
- 开发和验证一种高性能液体色谱-紫外线 (HPLC-UV) 方法,用于在人体血中量化卡博桑提尼布.
- 建立可靠的方法来监测卡博桑提尼布的治疗药物.
主要方法:
- 人体血样本用乙尼去蛋白化.
- 染色分离是在一个反向相柱上进行的,该柱有一个同位素移动相.
- 使用250nm紫外线探测器实现了检测.
主要成果:
- 在0.05-5μg/mL的度范围内,HPLC-UV方法证明了线性 (R2 = 0.99999).
- 测试准确度在 -4.35%至0.98%之间,回收率超过96.04%.
- 每个样本的总测量时间为9分钟.
结论:
- 开发的HPLC-UV方法对于在人体血中定量卡博桑提尼布是有效和准确的.
- 这种简单的方法适用于在临床环境中常规监测卡博桑提尼布水平.
- 优化监测可以提高治疗结果,并尽量减少卡博赞提尼布治疗患者的不良事件.
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