使用三明治免疫测试进行定量测量的安培罗米特生物传感器.
Thor Pedersen1,2, Peter Fojan1, Anne Kathrine Nissen Pedersen3
1Department of Materials and Production, Aalborg University, Skjernvej 4A, 9220 Aalborg, Denmark.
Biosensors
|May 26, 2023
概括
这项研究开发了一种新的电化学免疫传感器,用于在护理点快速检测生物标志物. 强大的传感器可以准确地检测尿液中的中性粒细胞凝酶相关脂素 (NGAL),提供便携且具有成本效益的诊断解决方案.
科学领域:
- 生物传感和电化学检测检测
- 用于诊断的生物标志物分析.
- 照顾点 (PoC) 技术
背景情况:
- 目前的临床免疫测试方法需要专门的设备,限制了点治疗 (PoC) 应用.
- 电化学生物传感器为分析生物流体中的生物标志物提供了便携式,经济高效的替代方案.
- 优化传感表面,固定和报告系统对于提高生物传感器性能至关重要.
研究的目的:
- 开发和验证一个强大的电化学免疫传感器,用于生物标志物检测.
- 为电化学传感平台进行酶相关免疫吸收测定 (ELISA) 的调整.
- 评估传感器在尿液中检测中性粒细胞凝酶相关性脂卡林 (NGAL) 的性能.
主要方法:
- 使用SEM和AFM对印和薄膜电极的表面特征.
- 针对电化学传感器格式的ELISA的调整.
- 在尿液中检测NGAL以评估传感器的稳定性和可重复性.
主要成果:
- 开发的电化学免疫传感器显示,NGAL的低检测极限为1 ng/mL.
- 实现了3.5-80 ng/mL的线性检测范围,变化系数 (CV%) 为8%.
- 该平台技术被证明适用于各种类型的电极上的基于免疫测试的传感器.
结论:
- 开发的电化学免疫传感器是PoC生物标志物检测的可行技术.
- 该传感器提供了一种可靠,可重复和灵敏的方法,用于尿液中NGAL量化.
- 这种平台技术可适应开发各种基于免疫测试的电化学传感器.
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