蛇形和形虫是自主生成的,以履行它们在虫翅膀中的功能
Xubo Zhang1, Yanan Ji1, Bernard Moussian2
1Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China.
Insects
|May 26, 2023
概括
酸脱乙酶 (CDAs) 对于昆虫的翅膀发育至关重要. 这项研究表明,果翅膀中的CDAs是局部产生的,并且不依赖脂肪体来形成适当的翅膀.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 昆虫生理学 昆虫生理学
背景情况:
- 第I组酸脱乙酶 (CDAs),CDA1 (蛇形,蛇形) 和CDA2 (形,形),对于昆虫皮层的形成和变形至关重要.
- 以前的研究表明,从脂肪体分泌的CDA1支持Drosophila melanogaster的气管发育.
- 翅膀组织中CDAs的来源 - 无论是局部还是系统的 - 仍然未确定.
研究的目的:
- 为了调查果翅膀中酸脱乙酶 (CDA) 是否在本地产生或来自脂肪体.
- 阐明CDA1 (蛇形) 和CDA2 (形) 在翅膀发育中的自主或非自主作用.
主要方法:
- 组织特异性RNA干扰 (RNAi) 用于向DmCDA1 (蛇形) 和DmCDA2 (形) 在Drosophila melanogaster的脂肪体和翅膀组织中的表达.
- 在基因抑制后进行了翅膀形态发生和透性的表型分析.
- 量化逆转录PCR (RT-qPCR) 用于评估脂肪体和翅膀组织中的基因表达水平.
主要成果:
- 在脂肪体中抑制蛇形和虫形并没有影响翅膀形态发生.
- 脂肪体内的RNAi自主减少了脂肪体内的蛇形和虫表达,但没有显示对翅膀表达的非自主影响.
- 在翅膀发育过程中抑制蛇形或虫形导致了翅膀形态和透性的缺陷.
结论:
- 发展中的果翅膀中蛇形和虫的产生是自主性的.
- 翅膀的发育独立于蛇形和虫的脂肪体衍生的酸脱乙酶.
- 这些发现澄清了CDAs对适当的翅膀皮质形成和完整性的本地要求.
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