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Uroš Glavinić¹, Milan Rajković¹, Marko Ristanić¹

¹Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia.

|May 26, 2023

概括

用于养蜂的提摩尔在较高度下对蜜蜂细胞产生基因毒性作用. 它没有防止过氧化引起的DNA损伤,这表明需要仔细应用.

科学领域:

环境毒理学环境毒理学
蜜蜂养殖科学蜜蜂养殖科学
细胞生物学细胞生物学

背景情况:

提摩尔是一种来自*Thymus vulgaris*的天然化合物,用于养蜂对抗*Varroa*虫.
它的安全性和对蜜蜂 (*Apis mellifera*) 的影响需要进行彻底的研究.
提摩尔的基因毒性和抗基因毒性潜力以前没有在蜜蜂细胞系中进行研究.

研究的目的:

评估蒂摩尔对*Apis mellifera*连续细胞系AME-711的基因毒性和抗基因毒性作用.
为了确定蜂蜜蜂细胞的安全和有害度.
评估蒂莫尔对抗氧化DNA损伤的保护能力.

主要方法:

彗星试验被用来检测暴露于蒂摩尔 (10,100,1000μg/mL) 的AME-711细胞中的DNA损伤.
特里潘蓝色排除试验证实了缺少甲基醇细胞毒性.
对抗原毒性进行了测试,通过与乙醇和过氧化 (H2O2) 共同化细胞进行了测试.

主要成果:

提摩尔在10μg/mL时没有诱导DNA损伤.
在提摩尔度为100和1000微克/毫升时观察到基因毒性作用.
蒂莫尔没有表现出抗原毒性作用,并且增强了H2O2诱导的DNA损伤.

结论:

在培养的蜜蜂细胞中,提摩尔在100μg/mL及以上的度下表现出基因毒性潜力.
乙醇不能防止H2O2诱导的DNA损伤,并且可能会加剧损伤.
建议仔细考虑在养蜂业中使用蒂摩尔,以减轻对蜜蜂的潜在不良影响.

关键词:

蜜蜂鸟 (Apis mellifera) 是一个有趣的动物.造成的DNA损伤是DNA损伤.连续细胞系连续细胞系.精油提取物精油提取物单细胞凝电泳是一种单细胞凝电泳.

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