九个线粒体基因组的Phasmatodea与两个小说线粒体基因重新排列和原始基因
Yani Yuan1, Lihua Zhang2, Ke Li1
1College of Life Science, Zhejiang Normal University, Jinhua 321004, China.
Insects
|May 26, 2023
概括
使用新的线粒体体对树枝和叶虫 (Phasmatodea) 的遗传学分析揭示了新的线粒体结构,并澄清了分类学关系,解决了一些长期存在的分类问题.
科学领域:
- 动物学 动物学
- 昆虫学 昆虫学是一门学科.
- 分子遗传学分子遗传学
背景情况:
- 杆状和叶状昆虫 (Phasmatodea序列) 的分类受到不明确的遗传关系和形态特征的融合进化所阻碍.
- 之前的研究缺乏足够的分子数据来解决Phasmatodea内的各种等级的分类学模两可.
研究的目的:
- 通过使用新测序的线粒体基因组 (线粒体基因组) 来研究Phasmatodea内部的遗传学关系.
- 为了识别和表征新的线粒体基因组结构和在树和叶子昆虫中的重组.
- 为Phasmatodea.的分类提供一个更强大的遗传学框架.
主要方法:
- 测序和分析了9个新的树和叶虫线粒基因组,范围从15,011bp到17,761bp.
- 鉴定基因转位 (例如trnR和trnA) 和新型线粒体结构 (例如12SrRNA-CR1-trnI-CR2-trnQ-trnM).
- 用贝叶斯推断 (BI) 和最大概率 (ML) 对来自56个Phasmatodean物种的线粒体蛋白质编码基因 (mtPCGs) 的遗传学分析.
主要成果:
- 在 *Carausis* 种类中发现了trnR和trnA转位,通过并联重复/随机损失 (TDRL) 模型解释.
- 在*Stheneboea repudiosa*中发现了一种新的线粒体结构,可能涉及控制区域内转移RNA基因的逆转和转移.
- 遗传学分析支持Lonchodinae和Necrosciinae的单一性,但表明Lonchodidae是多种性的. 斯马科是单种类的,克利特姆尼纳是类型的,而斯马科是Neophasmatodea的基底姊妹群.
结论:
- 该研究澄清了Phasmatodea内的几个子家族和家族的遗传学位置,解决了一些分类学上的不确定性.
- 新的线粒体基因组重排提供了对序列内的进化机制的洞察.
- 这些发现为树枝和叶子昆虫的分类提供了更稳定的系遗传基础.
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