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用Saccharomyces cerevisiae进行代谢工程,以实现高效的视网醇合成

Xuan Wang^1,2, Xianhao Xu^1,2, Jiaheng Liu^1,2

¹Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Journal of fungi (Basel, Switzerland)

|May 26, 2023

概括

研究人员对酵母进行了改造,使其产生高二醇,达到1.2g/L. 微生物合成的这一突破推动了用于工业应用的维生素A生产.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物生产微生物生产

背景情况:

乙醇 (维生素A) 对视力,免疫力和发育至关重要.
雷丁醇还表现出抗瘤特性,可以缓解贫血.
目前用于醇生产的方法面临局限性,需要新的方法.

研究的目的:

开发一种Saccharomyces cerevisiae菌株,用于高产量的视网醇生产.
为了建立一个基础,以工业规模制造雷丁醇.
优化微生物代谢途径,以增强维生素A的合成.

主要方法:

在Saccharomyces cerevisiae中构建了retinol的新合成途径.
进行了代谢网络的模块化优化,增加了视网醇标位.
采用传送器工程来管理细胞内前体积累.
选和半理性设计的关键酶,包括视网醇脱酶.
使用橄油进行双相提取发酵以提高回收率.

主要成果:

在摇瓶发酵中达到1.2g/L的最终网醇标位,创下历史新高.
通过代谢优化,从最初的3.6 mg/L增加到153.6 mg/L的视网醇产量.
通过酶工程进一步提高了网醇标位至387.4 mg/L.
通过使用工程酵母,证明了微生物成功生产视网醇.

结论:

经过工程改造的Saccharomyces cerevisiae菌株显示出对工业化视网醇生产的巨大潜力.
代谢和酶工程策略有效地提高了视网醇产量.
这项研究为可扩展和高效的维生素A合成提供了一个强大的平台.

关键词:

这种植物是Saccharomyces cerevisiae.橄油橄油的使用方法雷丁醇是一种可靠的药物.视网醇脱酶的使用维生素A是一种维生素A.

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