相关实验视频

Updated: Jul 29, 2025

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

基因组和转录组调查提供了对日病原体Phoma macdonaldii病原性的分子基础的见解

Xuejing Chen¹, Xiaoran Hao², Oren Akhberdi³

¹College of Biological and Geography Sciences, Yili Normal University, Yining 835000, China.

Journal of fungi (Basel, Switzerland)

|May 26, 2023

概括

对Phoma macdonaldii的基因组和转录组分析揭示了参与日黑茎疾病的关键毒性因素和代谢途径. 这项研究为了解病原和制定疾病管理策略提供了基础.

科学领域:

植物病理学植物病理学
菌类基因组学菌类基因组学
分子植物-微生物相互作用

背景情况:

福马马克多纳尔迪 (Phoma macdonaldii) 导致向日黑茎,这是一个重要的农业疾病.
了解P. macdonaldii病原性的分子机制对于疾病控制至关重要.

研究的目的:

为了执行基因组规模的组装和注释 Phoma macdonaldii.
为了研究日感染P. macdonaldii.期间的转录组变化.
为了确定参与P. macdonaldii病变的基因和途径.

主要方法:

整个基因组测序和P. macdonaldii的组装.
生物信息分析用于基因预测和功能注释 (CAZymes,毒性因子,二次代谢物).
RNA测序 (RNA-seq) 用于在不同阶段分析感染的向日组织中的基因表达.
定量实时PCR (RT-qPCR) 用于对差异表达基因 (DEG) 的验证.

主要成果:

麦当劳的基因组是38.24 Mb,有11,094个预测的基因,包括许多用于植物多糖体降解,病原体与宿主相互作用和毒性因子的基因.
转录组分析在感染期间发现了显著的差异表达基因 (DEG),特别是在代谢途径和二次代谢物生物合成中.
在不同感染阶段和组织中,包括与毒性因子,碳水化合物活性酶 (CAZymes) 和载体相关的371种上调的DEGs是常见的.

结论:

这是P. macdonaldii的第一个基因组规模报告,提供了全面的遗传资源.
已识别的基因和途径为向日黑茎病变的分子基础提供了洞察力.
该研究强调了开发新策略来管理P. macdonaldii疾病的潜在分子标.

关键词:

菲马马克多纳尔迪 (Phoma macdonaldii) 是一个黑色的树干,黑色的茎.太阳花,向日的使用.转录组 (transcriptome) 是一个转录组.病毒性基因是病毒性基因.进行全基因组测序.

更多相关视频

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

相关概念视频

Gene Flow

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.

Fungal Phylum Microsporidia

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...