基酸转移酶Rtt109 调节Monascus purpureus中的发育,形态发生和氨酸生物合成
Ruoyu Shi1,2, Pengfei Gong1, Qiaoqiao Luo1
1Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China.
Journal of fungi (Basel, Switzerland)
|May 26, 2023
概括
基酸转移酶Rtt109对于食用真菌Monascus的发展至关重要. 删除Rtt109会影响生长,增加Monascus颜料和氨酸,揭示其在真菌二次代谢中的作用.
科学领域:
- 菌生物学的真菌生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 基因酸转移酶 (HAT) 酶调节基因表达,对真菌生理学至关重要.
- 在食用真菌Monascus中HAT Rtt109的特定作用在很大程度上仍未被描述.
- 了解Rtt109的功能是控制Monascus二次新陈代谢的关键,包括色素和毒素的产生.
研究的目的:
- 为了识别和功能性地描述莫纳斯科的Rtt109基因.
- 调查Rtt109对Monascus发育,色素生产和氨酸生物合成的影响.
- 阐明Rtt109在Monascus二次新陈代谢中的调节机制.
主要方法:
- 使用CRISPR/Cas9.9进行基因鉴定和淘汰菌株构造.
- 对状虫的形成,殖民地生长和代谢物产生的表型分析.
- 实时定量PCR (RT-qPCR) 来评估基因表达水平.
主要成果:
- 删除Rtt109显著降低了形形成和殖民地生长.
- rtt109淘汰菌株表现出莫纳斯色素 (MPs) 和素 (CTN) 的产量增加.
- Rtt109显著影响了参与Monascus发育和二次新陈代谢的基因的转录表达.
结论:
- 组织素乙转移酶Rtt109在莫纳斯克的发育和二次新陈代谢调节中发挥着关键作用.
- Rtt109是一种关键调节剂,影响有益色素和真菌毒素citrinin.
- 调查结果为控制蒙纳斯工业应用中的氨酸含量提供了洞察力.
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