在Penicillium expansum中,Erg4参与了Ergosterol生物合成,结合和应激反应
Zhanhong Han1, Yuanyuan Zong1, Xuemei Zhang1
1College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
Journal of fungi (Basel, Switzerland)
|May 26, 2023
概括
虫有三个erg4基因 (erg4A,erg4B,erg4C) 参与了ergosterol的合成和分泌. 这些基因表现出功能冗余性,而erg4B删除对ergosterol水平和分泌物产生最大影响.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 埃尔戈斯特对真菌细胞膜至关重要.
- 在Penicillium expansum中erg4基因的功能以前是未知的.
- 丝状真菌依赖于埃尔戈斯特的生物合成途径.
研究的目的:
- 为了研究Penicillium expansum中的erg4基因的功能.
- 为了确定erg4A,erg4B和erg4C在ergosterol生物合成中的作用.
- 评估erg4基因删除对真菌生长,化和应激反应的影响.
主要方法:
- 在P. expansum. 中,基因删除和淘汰突变基因的产生.
- 对厄戈斯特醇水平的定量分析.
- 评估胞,胞形态和应激敏感性 (细胞壁完整性,氧化应激).
- 评估殖民地直径,发芽率和病原性.
主要成果:
- P. expansum拥有三个erg4基因:erg4A,erg4B和erg4C,而erg4B表达率最高.
- 删除突变体表现出 reduced ergosterol水平的降低,而erg4B删除具有最显著的影响.
- 在所有删除突变体中,胞减少,而erg4B和erg4C突变体表现出缺陷的胞形态.
- Erg4B和erg4C的缺失增加了对细胞壁和氧化应激的敏感性.
- 没有观察到对殖民地直径,子发芽,状体结构或致病性的显著影响.
结论:
- 在P. expansum中的erg4A,erg4B和erg4C基因表现出功能冗余.
- 这些基因对于厄戈斯特的合成和分泌是必不可少的.
- Erg4B和erg4C在子形态发生,细胞壁完整性和氧化应激反应中起到额外的作用.
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