相关实验视频

Updated: Jul 29, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

通过Malonyl-CoA通路从Yarrowia lipolytica中的葡萄糖中生产工程3-基烯酸

Shiyu Liu¹, Yao Sun¹, Tianhui Wei¹

¹School of Life Science and Technology, Harbin Institute of Technology, Harbin 150006, China.

Journal of fungi (Basel, Switzerland)

|May 26, 2023

概括

这项研究开创了使用*Yarrowia lipolytica**的微生物生产3-Hydroxypropionic酸 (3-HP). 工程酵母实现了16.23g/L的高产量,为3HP生物合成建立了新的基准.

科学领域:

生物技术和代谢工程生物技术和代谢工程
工业微生物学工业微生物学
合成生物学合成生物学

背景情况:

3-基酸 (3-HP) 是一个重要的工业化学中间体.
微生物合成为传统的化学生产方法提供了一种绿色替代方案.
*Yarrowia lipolytica* 是一个有前途的宿主,因为它对有机酸的耐受性和前体的可用性.

研究的目的:

为了高效地生产3HP,工程师 * Yarrowia lipolytica * .
在宿主生物体中识别和破坏3-HP降解途径.
建立一个基础的微生物平台,用于3-HP生物合成.

主要方法:

*Y. lipolytica*的基因工程包括基因过度表达 (*MCR-NCa*, *MCR-CCa*, *GAPNSm*, *ACC1*, *ACSSeL641P*) 和淘汰 (*MLS1*, *CIT2*).
识别和淘汰3HP降解基因 (*MMSDH*, *HPDH*).
振动小瓶和料批发发酵的优化.

主要成果:

用于3HP生产的重组*Y. lipolytica*菌株 (Po1f-NC-14) 的构建.
在摇瓶发酵中达到1.128g/L的3HP产量.
在料批发发酵中达到16.23g/L的显著更高产量.

结论:

这项研究代表了第一个成功的生产3-HP在Yarrowia lipolytica*.
与其他微生物宿主相比,开发的工程菌株表现出具有竞争力的产量.
为未来的工业规模3HP生产使用Y. lipolytica*提供了坚实的基础.

关键词:

3-基烯酸是3基烯酸中的一种.雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.降解降解降解降解降解.马洛尼尔 - 甲是一种

更多相关视频

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

Respiration Pathways

Respiration Pathways

Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...

Glycolysis

Glycolysis

Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...

Glycolysis: Preparatory Phase

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...