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长距离电气和信号引起的过氧化在physcomitrella

Mateusz Koselski¹, Sebastian N W Hoernstein², Piotr Wasko¹

¹Department of Plant Physiology and Biophysics, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, Lublin 20-033, Poland.

Plant & cell physiology

|May 26, 2023

概括

活性氧物种 (ROS) 在植物中触发电和信号,这对于远距离信息传输至关重要. 这项研究揭示了多氧化 (H2O2) 如何在中启动这些信号,影响基因表达和应激反应.

科学领域:

植物的信号传输.
蜂通讯蜂通讯是通过蜂通讯进行的.
生物物理学的生物物理.

背景情况:

电气和信号对于植物中长距离信息传输至关重要.
反应性氧物种 (ROS) 波与电信号和信号一起,在压力期间参与细胞间的细胞间通信.
有限的知识存在于ROS诱导的系统电/信号在Physcomitrella和它们的相互关系.

研究的目的:

调查ROS在模型Physcomitrella中唤起全身电气和信号的能力.
在这种植物模型中探索ROS,电信号和信号之间的关系.
了解这些信号在传输有关ROS存在的信息中的作用.

主要方法:

过氧化 (H2O2) 的外部应用来诱导信号.
使用 (通道抑制剂) 和EDTA (胆剂) 的抑制研究.
对与压力相关的基因的基因表达分析.
在原生细胞中使用GCaMP3生物传感器进行成像.

主要成果:

过氧化 (H2O2) 的应用诱导了长距离电信号 (膜电位变化) 在Physcomitrella.
信号生成是依赖的,由和EDTA抑制.
电信号显示部分依赖于谷氨酸受体 (GLR) 离子通道.
信号在减少的质子细胞中传播缓慢 (>5μm/s).
在H2O2治疗8分钟后,在遥远的组织中观察到与压力相关的基因的升级.

结论:

外部的H2O2可以引起Physcomitrella中的全身电气和信号.
这些信号是依赖的,并且部分涉及GLR通道.
ROS诱导的信号有助于在工厂内长途传输有关压力的信息.

关键词:

成像学成像学细胞激发性细胞激发性细胞对细胞的信号传递.谷氨酸受体的受体是什么过氧化是一种过氧化.与压力相关的基因

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