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优化胡卜产品准备有针对性的LC-MS/MS代谢分析

Olga Deda^1,2, Olga Begou^2,3, Helen Gika^1,2

¹Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

|May 26, 2023

概括

使用水友交互液体染色学-并列质谱法 (HILIC-MS/MS) 优化对 (Ceratonia siliqua) 粉和糖的样本制备,可以提高代谢物分析. 1:2的重量比与特定的pH调整被证明是最有效的准确的代谢分析.

科学领域:

* 食品科学和分析化学.
*专注于代谢学和生物活性化合物分析.

背景情况:

*胡卜 (Ceratonia siliqua) 是一种有价值的地中海作物,富含生物活性化合物.
* 胡卜产品具有潜在的健康益处,需要详细的化学分析.
*代谢学可以揭示胡卜的营养丰富特征.

研究的目的:

* 优化胡卜粉和糖代谢的样品制备方法.
*为了提高水友相互作用液体染色学-双重质谱 (HILIC-MS/MS) 分析的效率和质量.
* 确定最佳的提取条件,以最大限度地检测代谢物.

主要方法:

* 胡卜粉和糖样品的提取优化.
*各种参数:pH,溶剂类型和样品重量与溶剂体积比 (Wc/Vs).
*使用HILIC-MS/MS进行分析,并根据总面积和最大数量进行评估.

主要成果:

*1:2的Wc/Vs比率始终产生了最高数量的代谢物.
* 具有1:2 Wc/Vs比率的水性乙二对两种样本类型都有效.
* 优化的pH条件:基本的水性醇用于糖和酸性水性乙烯用于粉末.

结论:

*优化的样品制备协议显著改善了胡卜的代谢分析.
* 标准化方法对于高效的LC-MS/MS分析关至关重要.
* 这项研究为可重复和全面的胡卜化合物识别提供了基础.

关键词:

这就是HILIC-MS/MS.氨基酸是氨基酸中的一种.生物活性化合物生物活性化合物代谢产物的代谢产物优化的优化优化优化.样品的准备样品的准备

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