帕尼巴西勒斯农业菌种 (Paenibacillus agricola sp.) 是一个 从农业土壤中分离出来的nov
Hyosun Lee1, Dhiraj Kumar Chaudhary2, Oung Bin Lim1
1Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
Archives of microbiology
|May 26, 2023
概括
一种新型的细菌物种Paenibacillus agricola sp. Nov.,从农业土壤中鉴定出来. 这种运动,有氧细菌表现出独特的特征,使其成为Paenibacillus属内的独特物种.
科学领域:
- 微生物学 微生物学
- 细菌的分类学 细菌的分类学
- 农业微生物学 农业微生物学
背景情况:
- 从不同的环境中分离新型细菌菌株对于理解微生物多样性至关重要.
- 帕尼巴西勒斯 (Paenibacillus) 属包括一系列具有各种生态作用的细菌.
- 新分离物体的表征有助于扩大已知的细菌分类学.
研究的目的:
- 从农业土壤中分离和鉴定一种新型细菌菌株.
- 确定分离物与已知的物种的遗传学和基因组相关性.
- 根据综合数据,为新型隔离物提出一种新的物种名称.
主要方法:
- 从农业土壤中分离和培养细菌菌株S3N08T .
- 现型表征包括生长条件,格拉姆染色,触酶和氧化酶试验.
- 使用16S rRNA基因序列进行了家族遗传学分析.
- 基因组分析包括DNA G+C含量,平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
- 对menaquinone和极性脂质进行化学分类分析.
- 占主导地位的脂肪酸分析.
主要成果:
- 菌株S3N08T是一种白色的,棒状的,运动的,有氧的,格拉姆阳性细菌.
- 在10-40°C,0-1.0%NaCl和pH值6.5-8.0之间观察到最优的生长;触酶负,氧化酶阳性.
- 遗传学分析将S3N08置于Paenibacillus属内,其中Paenibacillus periandrae PM10T是最接近的亲属 (95.6%的16S rRNA相似性).
- 基因组数据 (ANI < 72.0%,dDDH < 19.0%) 证实了它与已知的物种的区别.
- 化学分类学数据 (MK-7,PG,PE,PGME) 和脂肪酸概况 (iso-C15:0,anteiso-C15:0,C16:0) 与一种新物种一致.
结论:
- 菌株S3N08T代表了Paenibacillus属内的新物种,基于综合的遗传学,基因组学,表型学和化学分类学证据.
- 这种新物种的建议名称是Paenibacillus agricola sp. 在新的一年里.
- 这种菌株的类型是S3N08T (KACC 19666T=NBRC 113430T).
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