莫雷拉·凯尼 (Moorella caeni) sp. sp. 的种类. nov.,从甲醇养反应堆的热友性无氧污泥中分离出来
Nicolás Vecchini Santaella1, Diana Z Sousa1, Alfons J M Stams1
1Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708WE Wageningen, Netherlands.
概括
菌株AMPT,此前被认为是*Moorella thermoacetica*,被重新归类为一种新物种,即*Moorella caeni* sp. 在新的一年里. 这种细菌重新分类是基于基因组基因组和表型分析.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 人类遗传学 是一个学科.
背景情况:
- 菌株AMPT此前根据16SrRNA基因序列相似性 (98.3%) 确定为*Moorella thermoacetica*.
- 这一初步分类表明,AMPT菌株与已确定的物种Moorella thermoacetica之间存在密切的关系.
研究的目的:
- 通过使用全基因组数据,对AMPT菌株进行全面的遗传学和分类学分析.
- 确定Moorella*属内的AMPT菌株的精确分类地位.
- 如果菌株AMPT代表一个独特的血统,提出一种新的物种名称.
主要方法:
- 使用全基因组序列的基因组基因组分析.
- 计算基因组相关性指数,包括数字DNA-DNA杂交 (dDDH) 和平均核酸标识 (ANI).
- 现型特征化以支持分类学差异化.
主要成果:
- 基因组基因基因分析表明,AMPT菌株代表了Moorella*属内的独特血统.
- 基因组相关性指数低于物种划分值:dDDH为52.2% (<70%) 和ANI为93.2% (<95%) 与*Moorella thermoacetica* DSM 521T相比.
- 遗传学和表型数据支持将AMPT菌株归类为新物种.
结论:
- 菌株AMPT (DSM 21394T=JCM 35360T) 并不是Moorella thermoacetica的一种菌株.
- 这些数据强烈支持在*Moorella*属内建立一种新物种.
- 这种新物种的拟议名称是 *Moorella caeni* sp. 在新的一年里.
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