病原体基因组学和基于菌体的解决方案,用于准确识别和控制沙门氏菌病原体
Angela V Lopez-Garcia1, Manal AbuOun1, Javier Nunez-Garcia1
1Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom.
Frontiers in microbiology
|May 26, 2023
概括
禽类中的多药耐药沙门氏菌构成了全球威胁. 这项研究结合了基因组学和菌体敏感性,以确定有效的生物控制,揭示了有希望的预防食品感染的战略.
科学领域:
- 食品微生物学和动物传染病监测.
- 食品传播病原体的基因组流行病学.
- 抗微生物耐药性和毒性因子分析.
背景情况:
- 沙门氏菌是一种重要的食源性病原体,经常与家禽有关,导致人类胃肠道感染.
- 全球越来越多的多药耐药 (MDR) 沙门氏菌蔓延需要加强控制策略.
- 了解基因组多样性对于追踪病原体传播和疾病贡献至关重要.
研究的目的:
- 在英国和泰国禽类沙门氏菌分离物中表征抗微生物耐药性基因和毒性因子.
- 通过使用长读测序来探索病毒性和耐药性之间的基因组联系.
- 为了确定孤立物对沙门氏菌菌体的敏感性,用于潜在的生物控制应用.
主要方法:
- 在143只家禽沙门氏菌分离物中对抗微生物耐药性和毒性基因的基因特征.
- 三个MDR分离物的长读测序,以调查毒性-耐药性相关性.
- 对22种特征性沙门氏菌菌体进行菌体敏感性测试.
主要成果:
- 沙门氏菌Typhimurium及其单相变种是最常见的血清病毒.
- 14-15%的分离物是多药耐药 (MDR),MDR菌株中毒性基因的高流行率.
- 菌体STW-77对关键的临床血清病毒,包括沙门氏菌和沙门氏菌Typhimurium,表现出广泛的溶解活性.
结论:
- 基因组分析确定了在家禽中普遍存在的全球流行性MDR克隆.
- 菌体敏感性测试显示,STW-77是对重要的沙门氏菌血清病毒的潜在生物控制剂.
- 结合基因组学和菌素敏感性测试,为禽类和食品链中的沙门氏菌控制提供了一个有希望的方法.
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