由BvrR/BvrS两组分系统控制的表型受到BvrR酸化的差异影响
Pamela Altamirano-Silva1, Jazmín Meza-Torres1, Ana Mariel Zúñiga-Pereira1
1Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
Frontiers in microbiology
|May 26, 2023
概括
布鲁塞拉流产BvrR/BvrS系统调节病毒毒性. 非化BvrR控制特定的基因,影响细菌生存和毒性因子表达不同于化BvrR.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 布鲁塞拉堕胎是一种动物传染病原体,需要细胞内生存才能产生毒性.
- BvrR/BvrS两组系统调节了诸如IV型分泌系统 (VirB) 和VjbR.等重要的毒性因子.
- BvrR酸化与DNA结合和基因转录控制有关,影响膜平衡和细胞内生存.
研究的目的:
- 研究BvrRS酸化状态在调节BvrRS控制的表型和基因表达中的作用.
- 要区分酸化与非酸化BvrR的调节机制.
- 了解BvrR酸化如何影响细菌毒性和膜特征.
主要方法:
- 产生了主要的阳性和阴性BvrR变体,模仿酸化和非酸化状态.
- 引入了野生型和变种BvrR到一个BvrR阴性Brucella abortus菌株中.
- 评估了BvrRS控制的表型 (多素耐药性,Omp25表达,细胞内存活率) 和蛋白质表达 (VjbR,VirB).
- 进行了促进体相互作用研究和全球转录分析.
主要成果:
- BvrR酸化状态对基因转录有不同的影响.
- 主要阳性和野生型BvrR恢复了多素耐药性和Omp25表达.
- 野生型和主导阳性BvrR,以及主导阴性变异,都恢复了细胞内生存,VjbR和VirB表达.
- 主导的负BvrR显示了与vjbR的特定促进体相互作用,但不是omp25,表明不同的调节作用.
结论:
- 根据其酸化状态,BvrR采用各种策略来进行转录控制.
- 非化BvrR直接影响病毒性相关基因的一个子集的表达.
- BvrR/BvrS系统的调节是复杂的,酸化状态决定了特定的基因标和下游表型.
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