人类乳头瘤病毒的存在及其在原发性乳头瘤中的物理状态
Leslye Sámano-Hernández1,2, Garfias Y3,4, Humberto González-Márquez2
1Microbiology and Ocular Proteomics, Research Unit, Institute of Ophthalmology Fundación de Asistencia Privada Conde de Valenciana, Chimalpopoca, 14 Colonia Obrera, 06800, México City, Mexico.
Heliyon
|May 26, 2023
概括
人类乳头瘤病毒 (HPV) DNA 仅检测到皮样本,而不是健康的结膜. 这项研究确定了HPV-11和HPV-18的基因型,表明HPV是HPV的基因型.
科学领域:
- 眼科医生 眼科 眼科
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 膜是一种常见的结膜过度生长,其发病因子仍有争议.
- 人类乳头瘤病毒 (HPV) 在膜发育中的作用是有争议的.
- 之前的研究表明,关于在pterygium中HPV存在的结果是相互矛盾的.
研究的目的:
- 在pterygium中调查HPVDNA的存在和基因型.
- 为了分析HPVDNA融入宿主细胞基因组.
主要方法:
- 聚合酶链反应 (PCR) 用MY09/MY11原始剂检测40个体和12个健康的结膜样本中的HPVDNA.
- 用DNA序列分析来识别HPV基因型.
- 西方斑点分析检测HPV-L1囊蛋白用于病毒整合评估.
主要成果:
- 在40个pterygium样本中的19个 (47.5%) 中检测到HPV DNA.
- 在12个健康的结膜样本中没有发现HPVDNA.
- 确定的HPV基因型是HPV-11 (11个样本) 和HPV-18 (8个样本).
- 在10个分析样本中,在3个样本中检测到HPV-L1囊蛋白,这表明病毒融合.
结论:
- 这项研究证实,HPV DNA的存在仅限于pterygium.
- 11号和18号HPV基因型与pterygium发育有关.
- 这些发现表明HPV在pterygium病原和病毒基因组整合中的潜在作用.
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