便微生物组分类学,多样性和功能潜力的差异在一组牛群中,试验中挑战了Mycobacterium avium亚种. 准结核病 (MAP) 是一种疾病
Chloe Matthews1,2, Aaron M Walsh2, Stephen V Gordon3
1Department of Biological Sciences, Munster Technological University, Bishopstown, T12 P928 Cork, Ireland.
Animals : an open access journal from MDPI
|May 27, 2023
概括
暴露于Mycobacterium avium亚种结核病 (MAP) 会改变牛的肠道微生物组,在挑战后3个月观察到的关键变化. 这些微生物组变化与免疫标记相关,为约翰氏病提供了潜在的诊断见解.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物免疫学 动物免疫学
- 大基因组学和微生物组分析.
背景情况:
- 约翰氏病是由Mycobacterium avium亚种结核病 (MAP) 引起的,是反动物的重大肠道疾病.
- 甲基因组学提供了先进的工具来研究复杂的胃肠道微生物组和病原体的影响.
- 了解微生物组的变化对于管理牛群中MAP感染至关重要.
研究的目的:
- 调查实验性挑战MAP的牛便微生物组的分类学和组成变化.
- 随着时间的推移,将暴露于MAP的牛的肠道微生物群与未暴露的对照组进行比较.
- 为了识别与MAP暴露相关的特定微生物物种和功能变化.
主要方法:
- 用MAP对牛进行实验的挑战.
- 从暴露 (n=35) 和对照 (n=20) 组中在注射后3,6和9个月收集便拭子样本.
- 便微生物群的元基因组分析,以评估分类学多样性和功能潜力.
主要成果:
- 在暴露于MAP和对照牛 (p <0.05) 之间观察到便微生物组合和功能的显著差异.
- 疫苗注射后3个月发生了主要的分类学和功能转变,甲生物菌,双菌和其他11个物种的丰度发生了变化.
- 微生物组变化与免疫病理学标记相关,包括miRNA-155,miR-146b和IFN-ɣ.
结论:
- 暴露于MAP显著影响牛便微生物组合.
- 在MAP挑战之后,特定的微生物物种和功能途径发生了改变.
- 鉴定的微生物组变化可以作为跟踪MAP暴露和兽医疾病进展的潜在生物标志物.
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