Mycobacterium avium 亚种. 这种细菌可以 来自乳制品山羊棚的不同环境样本中的结核病 - - 对结核病诊断和预防的采样策略的影响
Chris Pickrodt1,2, Karsten Donat2,3, Udo Moog3
1Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburger Straße 96a, 07743 Jena, Germany.
Animals : an open access journal from MDPI
|May 27, 2023
概括
环境采样可以有效检测出Mycobacterium avium亚种. 在乳制品山羊群中感染甲结核病 (MAP). qPCR证实了感染,而培养则为有针对性的控制计划确定了传播热点.
科学领域:
- 兽医微生物学 兽医微生物学
- 环境健康 环境健康
- 传染病流行病学 传染病流行病学
背景情况:
- 结核病是由Mycobacterium avium亚种引起的. 准结核病 (MAP),是畜牧业的一个重大问题.
- 环境污染是MAP传播的主要途径,特别是通过年轻动物的口服摄入.
- 有效的诊断策略对于在乳牛群中管理结核病至关重要.
研究的目的:
- 为了调查MAP的存在和分布,在一个感染结核,接种疫苗的乳母山羊群的谷仓环境中.
- 为了比较环境采样方法 (培养和qPCR) 对于MAP检测的有效性.
- 确定影响MAP检测和潜在传播地点的环境因素.
主要方法:
- 在8个时间点中从乳山羊群中采集了256个环境样本 (床铺,灰尘,料,水).
- 使用细菌培养和定量聚合酶链反应 (qPCR) 检查样品.
- 统计分析以比较检测率并确定与MAP确认相关的因素.
主要成果:
- 在所有样本类型 (117/256) 中使用qPCR检测到MAP DNA.
- 从28个床铺和1个独特的尘埃样本中成功培养了MAP.
- 积极的结果在高流量地区和室内季节更频繁.
- 通过MAP种植,Kidding笔被确定为潜在的感染源.
结论:
- 环境采样,特别是qPCR,对于确认MAP群体感染是有效的.
- 细菌培养为MAP传播的关键领域提供了宝贵的见解.
- 尘埃是MAP DNA检测的最佳选择,而床铺是MAP培养的最佳选择.
- 这些发现有助于制定有针对性的,特定于农场的结核病控制策略.
关键词:
约翰的疾病.这是一个MAP MAP.床上用品 床上用品控制程序 控制程序 控制程序尘埃尘埃的尘埃,就是我们的尘埃.环境采样环境采样动物料料料料料料小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的传输 传输 传输 传输 传输 传输水水水的水水的水更多相关视频
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