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在膀癌发育中的LncRNA差异表达

Lorenzo Spirito¹, Rufina Maturi², Sara Carmela Credendino²

¹Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania "Luigi Vanvitelli", Via De Crecchio 7, 80138 Naples, Italy.

Diagnostics (Basel, Switzerland)

|May 27, 2023

概括

这项研究确定了四种长非编码RNA (lncRNAs) 在膀癌 (BC) 中表达的变化. 这些新的 lncRNAs 显示出作为膀癌的诊断和随访标记物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

膀癌 (BC) 是一种常见的恶性瘤,尿癌占90%.
目前的诊断方法,如尿细胞学,在检测率上有局限性,并依赖病理学家的专业知识.
现有的BC生物标志物由于成本或敏感性较低而缺乏常规的临床实施.

研究的目的:

在膀癌中研究四种特定的长非编码RNAs (lncRNAs) 的表达:MPPED2-AS1,RMST,Khl14AS和PAR5.
探索这些lncRNAs作为BC诊断和监测的新生物标志物的潜力.

主要方法:

使用GEPIA数据库进行生物信息分析,以比较正常与癌症组织中的lncRNA表达.
定量逆转录聚合酶连锁反应 (qRT-PCR) 用于测量患者活检中的四个lncRNAs的表达.
对瘤性膀病变的分析,包括良性和恶性病例,从经历过尿道切除膀瘤 (TURBT) 的患者.

主要成果:

生物信息分析揭示了正常和癌症样本之间研究的lncRNAs的差异表达.
qRT-PCR证实了MPPED2-AS1,RMST,Khl14AS和PAR5在正常组织,良性病变和膀癌中的差异表达.
该研究在不同类型的膀病变中确定了这些lncRNA的独特表达模式.

结论:

这些发现强调了新型lncRNAs (MPPED2-AS1,RMST,Khl14AS,PAR5) 在膀癌的发展中的参与.
这些lncRNAs的改变表达可能会影响对BC进展至关重要的调节回路.
这项研究为评估lncRNA基因作为BC诊断和/或随访的潜在标记物提供了基础.

关键词:

膀癌:膀癌是一种癌症.基因表达的基因表达方式在 lncRNAs 中.

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