在正常生理条件下,人类尿液N-葡萄糖的微异质性和个体差异
Hend Zedan1, Kousuke Morimura1, Amr Elguoshy2
1Department of Life and Food Sciences, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.
Biomolecules
|May 27, 2023
概括
这项研究使用LC-MS/MS对人类尿液N-糖进行了全面分析,确定了109种N-糖. 这些发现为尿液N-甘氨酸分析和生物标志物发现提供了有价值的参考.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 尿液是生物标志物发现的丰富来源,因为它反映了全身和泌尿器官生理学.
- 尿液N-糖的详细分析是具有挑战性的,因为与自由寡糖相比,糖的丰度较低.
研究的目的:
- 通过使用先进的LC-MS/MS技术,彻底分析和量化人类尿中的N-葡萄糖.
- 建立人类尿液N-甘氨酸及其结构注释的参考资料.
主要方法:
- 使用氨酸释放的N-甘氨酸被标记为2-aminopyridine (PA).
- 在LC-MS/MS分析之前进行了离子交换分化.
- 使用LC-MS/MS进行了N-甘氨酸的鉴定和量化.
主要成果:
- 总共有109种N-甘氨酸在尿液中被识别和定量.
- 连续检测到58种N-甘氨酸 (≥80%的样本),占总信号的~85%.
- 大约50%的尿液N-糖可能来自脏/尿路,年龄和性别影响N-糖的丰度.
结论:
- 这项研究提供了人类尿液N-glycome的第一个全面的参考地图.
- 这些发现提供了关于尿液N-糖的起源及其作为生物标志物的潜力的见解.
- 尿液中的N-葡萄糖因年龄和性别而异的变化凸显了其动态性质.
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