在Shigella flexneri的上皮细胞入侵中MDR效流AcrAB的作用
Marco Coluccia1, Aude Béranger1, Rita Trirocco1
1Istituto Pasteur Italy, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Biomolecules
|May 27, 2023
概括
这种AcrAB-TolC对于Shigella flexneri的毒性至关重要. 删除AcrAB基因会损害细菌在上皮细胞中的入侵和生存,这揭示了这种排泄在Shigella病变发生过程中的新作用.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- AcrAB-TolC排泄是肠道细菌中主要的RND载体,有助于抗生素耐药性和毒性.
- 细菌性痢疾的致病原体Shigella是一种重要的人类病原体,其中AcrAB的作用不太清楚.
研究的目的:
- 研究AcrAB-TolC在Shigella flexneri的入侵和细胞内生存中的特定作用.
- 为了阐明AcrAB对Shigella病变的贡献.
主要方法:
- 创建了Shigella flexneri M90T菌株的基因删除突变 (acrA,acrB) 病毒.
- 在Caco-2上皮细胞内进行了细菌生存测试.
- 通过使用特定的EP抑制剂来抑制AcrB载体活性.
主要成果:
- 删除acrA和acrB基因显著降低了Shigella flexneri在上皮细胞内的存活率.
- 由于没有AcrAB,可以防止细菌从细胞传播到细胞传播.
- 证实了AcrB转运体活性对于皮内生存至关重要.
结论:
- AcrAB-TolC在Shigella flexneri的入侵和病原发生方面发挥着重要作用.
- 这种排泄对于细菌的生存和在宿主上皮细胞内传播至关重要.
- 这些发现扩大了AcrAB已知的功能,超越了抗生素耐药性,包括Shigella中的毒性机制.
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