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一种直接识别Cronobacter sakazakii在液体介质中的方法由MALDI-TOF MS提供

Danliangmin Song¹, Qunchao Su¹, Ai Jia¹

¹Department of Food Science, Northeast Agricultural University, Harbin 150038, China.

Foods (Basel, Switzerland)

|May 27, 2023

概括

一种新的液体检测方法增强了矩阵辅助激光吸附离子化飞行时间质谱 (MALDI-TOF MS) 以快速,准确地检测食源病原体Cronobacter sakazakii (C. sakazakii). 这种优化的技术可以提高各种样本的识别分数和定量潜力.

科学领域:

微生物学微生物学
分析化学分析化学
食品安全食品安全

背景情况:

矩阵辅助激光吸附离子化飞行时间质谱 (MALDI-TOF MS) 是微生物识别的关键技术.
克罗诺巴克特萨卡扎基 (C. sakazakii) 由于其高婴儿死亡率,在粉末婴儿配方 (PIF) 环境中构成重大威胁.
对于C. sakazakii的传统MALDI-TOFMS方法通常仅限于定性检测.

研究的目的:

开发和优化一种新的,具有成本效益的液体斑点预处理方法,用于MALDI-TOF MS.
为了提高C. sakazakii检测的准确性,可复制性和定量能力.
用响应表面方法确定液体检测方法的最佳参数.

主要方法:

开发一个低成本的液体斑点预处理协议.
使用响应表面方法优化预处理参数 (酸体积,超声波功率和时间,乙二体积).
在不同样本类型中验证方法的适用性,准确性和定量潜力.

主要成果:

确定了最佳参数:25微升70%的酸,350瓦超声波3分钟,75微升的酸.
获得了C. sakazakii (1926.42 ± 48.497) 的最高识别分数.
对70种C. sakazakii菌株的识别准确度达到了100%的证明.
在环境样本中确定的检测极限为4.1 × 10^1 cfu/mL,在PIF样本中为2.72 × 10^3 cfu/mL.

结论:

开发的液体检测方法为使用MALDI-TOF MS.识别C. sakazakii提供了强大的,准确的和可重复的方法.
这种方法显著改善了传统的定性检测,提供了定量潜力.
优化的协议适用于在关键的食品加工环境中检测C. sakazakii,如PIF生产.

关键词:

克罗诺细菌 sakazakiiii 的时间马尔迪-托夫 (MALDI-TOF) 是一个国家元首.直接识别直接识别液体斑点的发现液体斑点的发现响应表面方法的方法.

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