综合多谱和分子对接 了解发酵臭化合物和普通话鱼肌纤维蛋白之间的相互作用
Jia-Nan Chen1, Hui-Lin Zhao1, Yu-Ying Zhang1
1National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods (Basel, Switzerland)
|May 27, 2023
概括
这项研究揭示了特定的风味化合物如何与发酵食品中的肌纤维蛋白 (MP) 相互作用. 修改蛋白质的疏水性可以增强这些相互作用,潜在地改善食物的味道.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 感官分析 感官分析
背景情况:
- 香味化合物释放对于发酵食品的质量至关重要.
- 了解风味化合物和食物蛋白之间的相互作用是关键.
研究的目的:
- 研究四种关键发酵臭味化合物和肌纤维蛋白 (MP) 之间的结合相互作用.
- 阐明MPs在与这些化合物相互作用时的结构变化.
- 探索改善发酵食品风味的新策略.
主要方法:
- 多光谱学 (光火) 用于研究MP-化合物相互作用.
- 用分子对接模拟来分析结合力和复杂稳定性.
- 操纵了疏水性,以评估其对结合的影响.
主要成果:
- 所有四种测试的化合物 (英多尔,异乙酸,二甲基二硫化物,二甲基甲酸) 都与MPs结合,二甲基甲酸和二甲基二硫化物表现出更强的亲缘关系.
- 减少蛋白质的疏水性显著增强了这些结合相互作用.
- 相互作用主要涉及静态光火和通过键改变MP二次结构 (β-sheet到α-helix/随机线圈).
- 分子对接证实了通过键,范德瓦尔斯力和离子键形成的稳定复合体.
结论:
- 发酵-恶臭化合物与MPs的结合受到蛋白质疏水性的影响.
- 在化合物结合时发生MPs的结构变化,影响蛋白质构成.
- 针对疏水性相互作用提供了一种新的方法来调节风味化合物的结合,并提高发酵食品的质量.
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