由氧化纳米颗粒抑制IL-1β表达的基础机制
Ayaka Koga1,2, Chuencheewit Thongsiri3, Daisuke Kudo4
1Department of Health Sciences, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan.
Biomedicines
|May 27, 2023
概括
氧化纳米颗粒显示出抗炎作用,对细菌有 parodontopathic. 这些纳米粒子,NM80和NM300,降低了IL-1β等炎症标记物,这表明牙科材料的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 纳米技术纳米技术
背景情况:
- 氧化纳米颗粒因其生物活性和生物相容性而得到认可.
- 以前的研究表明,氧化纳米颗粒对口腔细菌具有杀菌性.
- 牙周病性细菌是牙周病炎症反应的关键贡献者.
研究的目的:
- 研究氧化纳米颗粒对炎症反应的生物效应.
- 评估不同尺寸的氧化纳米颗粒 (NM80/NM300) 对由牙周病变细菌诱导的炎症的影响.
主要方法:
- 类似巨细胞的J774.1细胞用来自*Aggregatibacter actinomycetemcomitans*的脂多糖 (LPS) 来刺激.
- 细胞与两种尺寸的氧化纳米颗粒 (NM80和NM300) 进行了联合处理.
- 测量了炎症反应,包括IL-1β表达和分泌;分析了信号通路 (PI3K/Akt,NF-κB,MAPK).
主要成果:
- 氧化纳米颗粒NM80和NM300都抑制了LPS诱导的IL-1β表达和分泌.
- NM80的抗炎作用涉及PI3K/Akt介导的NF-κB激活和MAPK酸化的下调 (JNK,ERK1/2,p38).
- NM300的IL-1β抑制与ERK1/2信号通路的失活有关.
结论:
- 氧化纳米颗粒表现出显著的抗炎作用,对抗病性细菌因子.
- 纳米颗粒的大小会影响其抗炎作用背后的特定分子机制.
- 这些发现支持氧化纳米粒子在开发先进牙科材料方面的潜在应用.
相关概念视频
NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...


