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血红蛋白β表达与清细胞细胞癌的预后不佳有关

Yuta Kurota¹, Yuji Takeda², Osamu Ichiyanagi¹

¹Department of Urology, Faculty of Medicine, Yamagata University, 2-2-2, Iida-Nishi, Yamagata 990-9585, Japan.

|May 27, 2023

概括

血红蛋白β链 (HBB) 表达通过促进癌细胞增殖,使清细胞细胞癌 (ccRCC) 的预后恶化. 减少HBB增加了活性氧物种 (ROS),这表明HBB是ccRCC的潜在预后生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

瘤微环境的氧化还原平衡对于癌细胞适应缺氧至关重要.
血球蛋白β链 (HBB) 清除活性氧物种 (ROS),并且在癌症中发现.
对于HBB在细胞癌 (RCC) 的预后意义尚不清楚.

研究的目的:

研究HBB表达在清细胞细胞癌 (ccRCC) 预后中的作用.
确定HBB对ccRCC细胞行为和ROS生产的功能影响.

主要方法:

在203个非转移性ccRCC病例中对HBB表达的免疫组织化学分析.
在体外实验中,使用用HBB特异性siRNA处理的ccRCC细胞系来评估扩散,入侵和ROS水平.
在由H2O2.2.引起的氧化应激下评估HBB表达变化.

主要成果:

与HBB阴性患者相比,HBB阳性ccRCC患者的预后较差.
使用siRNA的HBB敲除显著抑制了ccRCC细胞的增殖和入侵.
降低HBB导致ccRCC细胞中的ROS产量增加.
由CCRCC细胞中H2O2上调HBB表达引起的氧化应激.

结论:

在ccRCC中HBB表达通过抑制ROS的产生,特别是在低氧条件下,促进癌细胞的增殖.
HBB表达与ccRCC中的不良临床结果有关.
HBB显示出作为细胞癌的新预后生物标志物的潜力.

关键词:

血红蛋白β链 (HBB) 是一种恶性转变的恶性转变氧化还原平衡的重氧化平衡.细胞癌 (RCC) 是一种细胞癌.

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