基因表达概况的动力学和重症COVID-19高风险患者的鉴定
Alexander Rombauts1, Marta Bódalo Torruella2, Gabriela Abelenda-Alonso1
1Department of Infectious Diseases, Hospital Universitari de Bellvitge-IDIBELL, 08908 Barcelona, Spain.
Biomedicines
|May 27, 2023
概括
主体对SARS-CoV-2感染的反应显著影响COVID-19的严重程度. 这项研究揭示了在患有急性呼吸困扰综合征 (ARDS) 的患者中明显的全血转录形状,突出显示了免疫失调和表观遗传因素.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 感染SARS-CoV-2的临床结果从无症状到致命,宿主反应是关键决定因素.
- 了解COVID-19患者反应的异质性对于预测疾病进展和开发向疗法至关重要.
研究的目的:
- 为了调查住院成人COVID-19患者的动态全血转录组资料.
- 描述急性呼吸困扰综合征 (ARDS) 患者和没有发展的患者之间的转录组差异.
- 阐明严重COVID-19和ARDS背后的宿主反应机制.
主要方法:
- 招募了60名住院COVID-19患者,其中19人患有ARDS.
- 在入院和第7天使用PAXGene RNA管收集了外周血液样本.
- 分析了全血转录基因特征,以确定差异表达的基因.
主要成果:
- 在ARDS患者与非ARDS患者相比,在基线确定了2572个差异表达基因,在第7天确定了1149个.
- 在ARDS患者中观察到失调的炎症反应,包括增加的促炎分子,中性粒细胞和巨细胞激活,以及免疫调节损失.
- 在ARDS的后期阶段发现了与活性氧物种,蛋白质多化和金属蛋白酶相关的基因的高表达.
- 突出了长非编码RNA在表观遗传控制中的作用,作为ARDS和非ARDS患者之间的显著差异化因素.
结论:
- 全血转录基因分析显示,在患有ARDS的COVID-19患者中,有不同的宿主反应模式.
- 早期的免疫失调和后来的氧化应激/矩阵重塑途径都与ARDS的发病有关.
- 长非编码RNA和表观遗传修饰代表了严重COVID-19的潜在生物标志物和治疗点.
相关概念视频
02:51mRNA Stability and Gene Expression
5.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.7K
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K