Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Next-generation Sequencing03:00

Next-generation Sequencing

91.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Astroglia Syncytial Theory of Consciousness.

International journal of molecular sciences·2025
Same author

DNA Barcoding and Metabarcoding Protocols for Species Identification.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Guidelines for the Analysis of DNA Barcoding/Metabarcoding Sequencing Data and Interpretation of Publicly Available Databases.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Assessment of detection limits for dyed and mounted textile fibers using Raman spectroscopy.

Journal of forensic sciences·2022
Same author

Recovery of DNA from fired and unfired cartridge casings: comparison of two DNA collection methods.

Forensic science international. Genetics·2022
Same author

Rapid, presumptive identification of seed-based toxins using direct analysis in real time mass spectrometry (DART-MS) and its variants.

Science & justice : journal of the Forensic Science Society·2022

相关实验视频

Updated: Jul 29, 2025

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.6K

对不同样本类型的图书馆准备工作流程和应用程序的评估使用PowerSeq® 46GY系统与大规模并行测序.

Kyleen Elwick1, Patrick Rydzak1, James M Robertson2

  • 1Visiting Scientist Program, Research & Support Unit, Laboratory Division, Federal Bureau of Investigation, 2501 Investigation Parkway, Quantico, VA 22135, USA.

Genes
|May 27, 2023
PubMed
概括

该PowerSeq 46GY系统,及其制造商.

关键词:
在PowerSeq® 46GY系统中.这是STRs.在图书馆,图书馆.图书馆准备工作 图书馆准备工作大规模并行测序 (MPS)下一代测序 (NGS) 是指下一代的测序.净化珠子 净化珠子

更多相关视频

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

相关实验视频

Last Updated: Jul 29, 2025

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.6K
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

科学领域:

  • 法医科学 法医科学 法医科学
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • 法医DNA分析依赖于DNA分析的准确和高效方法.
  • 优化图书馆准备和量化对于最大限度地提高下一代测序 (NGS) 基于法医应用的数据质量至关重要.
  • PowerSeq 46GY系统是一个用于法医DNA分析的原型NGS平台.

研究的目的:

  • 评估PowerSeq 46GY系统的原型,用于法医DNA分析.
  • 评估协议修改对读取覆盖范围和样本分析的影响.
  • 为了比较不同的图书馆准备套件和量化方法.

主要方法:

  • 使用捐赠者DNA和案例样本评估PowerSeq 46GY系统.
  • 使用TruSeq DNA PCR-Free HT和KAPA HyperPrep套件准备口腔和案例类型的图书馆.
  • 比较PowerSeq Quant MS系统和KAPA图书馆量化套件,以及KAPA大小调整工作簿.
  • 在MiSeq FGx平台上进行测序,并使用STRait Razor进行数据分析.

主要成果:

  • 所有评估的量化方法都高估了图书馆度;PowerSeq Quant MS系统显示了最高的准确性.
  • 与KAPA套件相比,TruSeq图书馆套件产生了更好的结果,包括更高的覆盖率和更少的等位基脱落.
  • 骨头和头发样本显示完全恢复,骨头样本的平均覆盖率更高.

结论:

  • 与其他图书馆准备方法相比,PowerSeq 46GY系统的制造商协议提供了最好的整体结果.
  • 优化图书馆准备和准确的量化是使用NGS技术成功进行法医DNA分析的关键.
  • 该PowerSeq 46GY系统展示了可靠的法医DNA分析的潜力,即使是具有挑战性的样本类型.