在人类呼吸道共囊病毒和人类博卡病毒感染中,炎症媒介的分子剖析

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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

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Noorah A Alkubaisi¹, Ibrahim M Aziz¹, Asma N Alsaleh¹

¹Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

|May 27, 2023

概括

细胞因子概况,包括IL-6和IL-8,在患有人类呼吸道同胞病毒 (HRSV) 和人类口腔病毒 (HBoV) 感染的儿童中发生变化,与疾病严重程度相关. 与HRSV和HBoV的同时感染显示出明显的细胞因子升高.

科学领域:

儿童传染病儿童传染病
免疫学免疫学免疫学
病毒学病毒学

背景情况:

人类呼吸道同胞病毒 (HRSV) 和人类口腔病毒 (HBoV) 感染在儿童中很常见,并可能导致严重的呼吸道疾病.
像IL-6,IL-8和TNF-α这样的亲炎性细胞因子与这些病毒感染的发病和严重性有关.
了解细胞因子表达模式对于治疗儿科呼吸道感染至关重要.

研究的目的:

为了研究和比较住院儿童与HRSV,HBoV或共感染的细胞因子和化学因子表达特征.
确定特定细胞因子水平与这些病毒感染中的疾病严重程度之间的关联.

主要方法:

通过rRT-PCR对75个被诊断患有HRSV,HBoV或共感染的儿童的鼻吸液 (NPA) 进行分析.
使用定量PCR (qPCR) 测量各种细胞因子和化学因子的表达水平.
患者组和对照组之间的细胞因子水平的比较,以及轻度和严重感染病例之间的比较.

主要成果:

与对照组相比,在感染儿童中观察到IL-6,IL-8,IL-10,IL-13,IL-33和G-CSF的升高水平.
患有HRSV和HBoV共感染的儿童显示IL-4,IL-17,GM-CSF和CCL-5的水平明显更高.
严重的HRSV感染与TNF-α,IL-6,IL-8,IL-10,IL-13和IL-33的增加有关,而严重的HBoV感染显示IL-10,IL-13和IL-33的增加.

结论:

显著的细胞因子和化学因子配置与HRSV,HBoV以及儿童的共感染有关.
特定细胞因子的升高与单一和共感染的疾病严重程度相关.
需要进一步的研究,以阐明这些细胞因子在HRSV和HBoV感染病变发生过程中的确切作用.

关键词:

这就是HRSV.人类口腔病毒人类口腔病毒人类骨架肺炎病毒有助于炎症的细胞因子.

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