相关实验视频
Updated: Jul 29, 2025

13:33
Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
39.1K
开发和验证一个54K全基因组液态SNP芯片面板,用于乳制品山羊的目标测序
Shengyu Guan1, Weining Li1, Hai Jin2
1State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Genes
|May 27, 2023
概括
研究人员为奶羊开发了一种液体SNP芯片面板,使精确的基因组选择成为可能. 这种工具确定了一种与外套颜色相关的关键基因,提高了山羊繁殖效率.
科学领域:
- 动物遗传学动物遗传学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 单核酸多态 (SNP) 芯片对于牲畜的基因组选择至关重要.
- 开发具有成本效益和高通量的基因型识别平台对于乳山羊育种计划至关重要.
研究的目的:
- 开发和验证一种新的液体SNP芯片面板,用于乳山羊.
- 利用全基因组关联研究 (GWAS) 的小组来识别影响毛皮颜色等特征的基因.
主要方法:
- 通过针对性测序 (GBTS) 技术的基因型鉴定被用来创建一个由54,188个SNP组成的小组.
- 该小组使用来自不同品种的110只乳山羊的全基因组再测序数据开发.
- 通过对200只山羊进行基因型鉴定来评估小组的表现,并通过对15只个体的全基因组再测序来验证.
主要成果:
- 液体SNP芯片面板实现了98.41%的平均捕获率和98.02%的基因型一致性,全基因组重新排序.
- 使用该小组的GWAS发现了8号染色体上外套颜色的显著关联信号.
- 已知影响外套颜色的TYRP1基因位于已识别的显著基因组区域内.
结论:
- 开发的液体SNP芯片面板为乳牛山羊基因组学提供了高精度和成本效益.
- 这个平台可以显著增强遗传分析,提高乳山羊种群的繁殖效率.
- 鉴定TYRP1与毛皮颜色的关联,为山羊养殖提供了宝贵的遗传信息.
相关概念视频
Single Nucleotide Polymorphisms-SNPs
15.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
Genome-wide Association Studies-GWAS
13.7K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
13.7K

