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相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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相关实验视频

Updated: Jun 22, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
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An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

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多复合基因工程基于dCas9和gRNA-tRNA数组编码在单个转录.

Chaoqian Jiang1,2, Lishuang Geng1,2, Jinpeng Wang1

  • 1Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.

International journal of molecular sciences
|May 27, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了基于CRISPR的多重基因组工程平台,使多个基因的同时调节成为可能. 该系统为复杂的生物研究提供了转录控制和表观遗传修饰.

关键词:
这就是CRISPR/Cas9的作用.多重基因组工程是多重基因组工程.这是一个tRNA-gRNA数组.三倍数序列的三倍数序列.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学是一种遗传学.
  • 合成生物学 合成生物学

背景情况:

  • 多复合基因组工程对于理解基因相互作用和遗传网络至关重要.
  • 同时针对多个基因组位点,可以对复杂的表型进行全面分析.

研究的目的:

  • 开发一种基于CRISPR的多功能平台,用于同时进行多重复合的基因组工程.
  • 为了使多个功能 (激活,抑制,甲基化,脱甲基化) 在多个基因组位点从单个转录.

主要方法:

  • 融合RNA发针 (MS2,PP7,com,boxB) 用于引导RNA支架和相关RNA结合蛋白到作用器.
  • 在单个转录上构建多个导向RNA,并列排列转移RNA-导向RNA架构.
  • 集成的三重序列用于同时表达蛋白质和RNAs.

主要成果:

  • 通过配对的RNA毛刺和RNA结合蛋白,证明了多个基因的同时独立调节.
  • 成功地应用了转录激活,抑制,DNA甲基化和DNA脱甲基化在内源性点上的系统.
  • 利用多达16个单独的CRISPR指导RNA在单个转录中交付.

结论:

  • 开发的平台为研究复杂的合成生物学问题提供了强大的工具.
  • 该系统可为医疗应用设计复杂的表型.
  • 提供了一种用于同时,多重化基因组工程的新方法,具有多样化的功能输出.