在流感A病毒感染期间PD-L1的免疫抑制作用
Hongya Ning1, Shih-Hsin Chiu1, Xiaodong Xu1
1Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences (College of Bee Science), Fujian Agricultural and Forestry University, Fuzhou 350002, China.
International journal of molecular sciences
|May 27, 2023
概括
编程死亡配体1 (PD-L1) 的表达在H1N1流感A病毒 (IAV) 感染期间增加,促进病毒复制. 针对PD-L1可能为流感A病毒感染提供新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 编程死亡配体1 (PD-L1) 是癌症免疫治疗的目标.
- 它在病毒感染,特别是流感中的作用不太清楚.
- PD-L1可以调节免疫反应和细胞过程.
研究的目的:
- 为了研究PD-L1在H1N1流感A病毒 (IAV) 感染中的作用.
- 探索在IAV感染期间PD-L1和SHP2信号传递之间的关联.
- 评估PD-L1作为流感的潜在治疗点.
主要方法:
- 评估了PD-L1对H1N1IAV感染的反应的升级.
- 研究了PD-L1过度表达对病毒复制和干扰素途径的影响.
- 使用SHP2抑制剂 (SHP099,siSHP2) 和SHP2过度表达来分析PD-L1/SHP2相互作用.
- 测量了p-ERK和p-SHP2的表达在PD-L1过度表达的细胞中.
主要成果:
- H1N1 IAV感染显著提高了PD-L1表达的调节.
- 过度表达PD-L1增强了病毒复制,抑制了I/III型干扰素和ISG.
- 抑制SHP2降低了PD-L1的表达,而SHP2的过度表达增加了它.
- PD-L1过度表达减少了WSN/PR8诱导的p-SHP2和p-ERK表达.
结论:
- 在H1N1 IAV感染期间,PD-L1在免疫抑制中发挥着重要作用.
- PD-L1 影响病毒复制和干扰素反应.
- PD-L1/SHP2轴与H1N1 IAV病原发生有关.
- PD-L1代表了新型抗流感药物的潜在治疗标.
更多相关视频
09:26In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Published on: October 15, 2013
14.1K
10:18Author Spotlight: Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 Interaction
Published on: July 7, 2023
1.3K
相关概念视频
01:29Immune Response Against Viral Pathogens
837
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
837
02:28Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K