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虹膜/FNDC5表达及其血清水平在乳腺癌中的作用

Kamil Cebulski¹, Aleksandra Piotrowska¹, Alicja Kmiecik¹

¹Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.

International journal of molecular sciences

|May 27, 2023

概括

虹素 (Ir) 是由纤维素第3类域含有蛋白5 (FNDC5) 衍生而来的,在乳腺癌中表达变化. 较高的血清Ir水平与转移和等级相关,而组织FNDC5/Ir与E-cadherin表达有关.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
生物化学生物化学

背景情况:

虹膜素 (Ir) 是由纤维素第3类域含蛋白5 (FNDC5) 衍生而来的肌素,它与各种生理过程有关.
在乳腺癌 (BC) 中,人们对FNDC5/Ir在抑制上皮层-介质细胞过渡 (EMT) 的作用知之甚少.
了解FNDC5/Ir表达及其与BC中EMT标记物的关系对于治疗开发至关重要.

研究的目的:

研究乳腺癌组织和细胞系中FNDC5/Ir的细胞局部化和表达水平.
为了比较血清Ir水平与BC组织中的FNDC5/Ir表达.
为了分析FNDC5/Ir表达和EMT关键标记物 (E-cadherin,N-cadherin,SNAIL,SLUG,TWIST) 在BC.之间的相关性.

主要方法:

在541个BC样本的组织微阵列上对FNDC5/Ir和EMT标记物的免疫组织化学分析.
77名BC患者血清Ir水平的评估.
在BC细胞系 (MCF-7,MDA-MB-231,MDA-MB-468) 和正常乳腺细胞系 (Me16c) 中FNDC5/Ir表达和局部化的体外分析.

主要成果:

在BC细胞和瘤纤维细胞的细胞质中检测到FNDC5/Ir,BC细胞系中的表达率高于正常细胞.
血清Ir水平与组织FNDC5/Ir表达没有相关性,但与淋巴结转移和组织学级相关.
FNDC5/Ir表达与E-cadherin和SNAIL有中等的相关性,与E-cadherin水平有积极的关联.

结论:

在乳腺癌组织和细胞系中存在FNDC5/Ir,表达水平在正常细胞和恶性细胞之间有所不同.
血清Ir水平作为晚期疾病的潜在生物标志物,表明淋巴结转移和更高的瘤等级.
FNDC5/Ir表达与E-cadherin有关,这表明它在乳腺癌中调节EMT过程中的作用.

关键词:

这是E-cadherin.在EMT中,EMT是EMT.在FNDC5中,FNDC5是FNDC5.一个叫做slug的slug.一个的子这是一个扭曲的世界.乳腺癌 (BC) 是一种虹膜是一种虹膜.

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